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applications
- protein purification:
– desalination, buffering, removal of dyes and concentration of samples (e.g. protein synthesis) - renaturation / denaturation of proteins:
– removal of urea, removal of guanidine hydrochloride - cleaning of nanoparticles
- equilibrium dialysis
– hormone determination (e.g. testosterone)
– interaction and binding studies of active substances - Cell-free protein synthesis
advantages
- high sample throughput with simultaneous reduction of analysis and sample preparation times by 2 to 12 times
- high yield with up to 98% sample recovery
- optimized for use in liquid handling systems according to the microtitre plate standard
- easy sample loading and sample extraction with standard laboratory pipettes
- autoclavable
Sample dialysis – accelerated & controllable
The Xpress Dialyzers are ready-to-use dialysis systems for small sample volumes. They enable very fast and efficient purification of protein, RNA and DNA samples. Compared to ultrafiltration and chromatography, the dialysis cartridges have excellent sample recovery, offer gentle sample handling and are very convenient to use.
The unique geometry of the scienova dialysis cartridges, the optimal ratio of membrane area to sample volume and at the same time a minimal diffusion path, ensures a significant shortening of exchange and interaction processes (dialysis time).
Designed in practical cartridge format, the scienova Xpress Dialyzers are designed “ready-to-use” and allow easy handling with conventional laboratory pipettes.
Typical applications of the Xpress Dialyzers are, for example, the purification of protein samples after tryptic digestion prior to LC/MS analysis or the purification of unwanted substances after protein synthesis.
The scienova Xpress Dialyzers are compatible with the microtiter plate standard (SBS) and can be used in most pipetting and laboratory automation systems. This makes them particularly suitable for high-throughput screening (HTS) or scouting experiments.
Interaction and binding studies, for example in pharmaceutical drug development or in the diagnostic determination of free hormones (e.g. testosterone) from blood serum, can be performed with equilibrium dialysis.
With this modern dialysis technology, you not only conserve your resources, but also gain a completely new quality of cost-effectiveness compared to conventional separation methods. Small sample volumes, a large number of samples and safe handling – no classical dialysis tube can compete with this.
Xpress Dialyzer Family
Membrane material low-binding regenerated cellulose I exclusion quantities (MWCO): 2, 3.5, 6-8, 20 kDa I temperature: 1-60°C I pH-value: 4-8
Membrane material Biocellulose Bio-Xell ® I exclusion quantities: 2 I 3.5 | 6–8 | 12–14 I 20 I 140 kDa I temperature: 1-60°C I pH-value: 5-8
MD100
Sample volume 10-100 μl
Deepwell plates 96-well
ED300
Sample volume 50-300 μl
Deepwell plates 96-well
MD1000
Sample volume 150-1000 μl
Deepwell plates 48-well
MD300
Sample volume 50-300 μl
Deepwell plates 96-well
XDR
Sample volume 1 ml – 19 ml
Deepwell plates 6-well
Xpress Micro Dialyzer MD100
The Micro Dialyzer MD100, the oldest family member of the scienova Xpress Dialyzer, is suitable for smallest volumes from 10 to 100 µl and captivates with a sample recovery of up to 98%. Whether protein purification, buffering or renaturation, the fields of application are broad.
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- Sample volumes between 10 and 100 µl
- Cut-off 2 I 3.5 | 6-8 | 12-14 I 20 I 140 kDa
- SBS compatible
- Autoclavable
- Up to 98% sample recovery
- Protein and peptide sample purification
- Sample renaturation
- Buffering of protein samples
- In vitro translation
- Enzyme activity studies
Xpress Micro Dialyzer MD300
The Micro Dialyzer MD300 is used for the preparation of small to medium sized samples between 50 and 300 µl. Like all Xpress Dialyzers, it is autoclavable and is available with different dialysis membranes of different cutoff sizes.
- Sample volumes between 50 and 300 µl
- Cut-off 2 I 3.5 | 6-8 | 12-14 I 20 I 140 kDa
- SBS compatible
- Autoclavable
- Protein and peptide sample purification
- Sample renaturation
- Buffering of protein samples
- In vitro translation
Xpress Equilibrium Dialyzer ED300
The ED300 Equilibrium Dialyzer has a modified geometry that is particularly user-friendly. It has 2 channels, one for sample processing and the other for buffer exchange. This ensures time-saving handling and high recovery of dialyzed samples as well as trouble-free removal of the dialysis buffer. It is designed for sample volumes between 50 and 300 µl. Based on this technology, only larger, the MD1000 Mini Dialyzer was also developed.
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- Sample volumes between 50 and 300 µl
- Cut-off 3.5 | 6-8 | 12-14 I 20 I 140 kDa
- 2-channel system allows easy sample handling and buffer change directly from the deep well plate
(automatable process) - SBS compatible
- Autoclavable
- Protein and peptide sample purification
- Sample renaturation
- Buffering of protein samples
- In vitro translation
- Enzyme activity studies
- Binding studies (hormones, drugs)
Xpress Mini Dialyzer MD1000
The MD1000 Mini Dialyzer, suitable for volumes between 150 and 1000 µl, impresses with its unique geometry, which enables particularly short dialysis times and is user-friendly. Its 2-channel system allows you to pipette your samples and process the buffer through a second channel. This flexibility saves time and makes it particularly suitable for equilibrium dialysis (e.g. for the determination of hormones in serum or for the production of antigens).
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- Sample volumes between 150 and 1000 µl
- Cut-off 2 I 3.5 | 6-8 | 12-14 I 20 I 140 kDa
- 2-channel system allows easy sample handling and buffer change directly from the deep well plate (automated process)
- Protein and peptide sample purification
- Sample renaturation
- Buffering of protein samples
- In vitro translation
- Enzyme activity studies
Xpress Dialysis Reactor XDR
The XDR (Xpress Dialysis Reactor) has a much larger capacity than its siblings. It can be used for sample volumes between 1 and 19 ml and can be placed in a floater or deep-well plate for dialysis just like the other Xpress Dialyzers. It also has a special lid that offers various connection options. This modular design provides additional flexibility in the processing of your samples and increases the range of applications. So you can not only dialyze your samples, but also ventilate or mix them.
- Sample volumes from 1 ml – 19 ml
- Cut-off 2 I 3.5 | 6-8 | 12-14 I 20 kDa
- Luer-Lock connection for hose systems
- Can be used in deep well plates or floaters
- Liquid handling with disposable syringes
- Circulation and aeration of samples with peristaltic pump
- Sample mixing with disposable syringes
- Dialysis
Xpress Magnetic Mixing Dialysis Box
The Xpress Magnetic Mixing Box has been developed as an additional tool for the MD100, the MD300 and the ED300. It is particularly suitable for dialyzing small volume samples against a large uniform buffer volume. To make this faster, the enclosed magnetic stirring rod circulates the dialysis buffer when used on a magnetic stirrer.
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- Even higher dialysis efficiency through buffer circulation during dialysis
- Complete set with 12 cartridges of Xpress Mirco Dialyzers 100 / 300 or Xpress Equilibrium Dialyzers
- Configured for immediate use on a magnetic stirrer
- Protein and peptide sample purification
- Sample renaturation
- Buffering of protein samples
GridKit48
- Larger buffer volume
- Convenient buffer change directly in the deep well plate due to positioning grid
- Optimized and tested for use in liquid handling systems
- Protein and peptide sample purification
- Sample renaturation
- Buffering of protein samples
- In vitro translation
- Enzyme activity studies
