Content: 1 g
AppliChem No: A1421 (A1421,0001)
CAS: 30827-99-7
Technical data:
Solubility: 50 g/L (H2O)
Physical Description: Solid
Storage: 2-8 °C
Specifications:
Assay (titr., calc. on dried substance): min. 98 %
Assay (HPLC): min. 98 %
Loss on drying: max. 1.0 %
AEBSF irreversibly inactivates thrombin and other serine proteases (e. g. chymotrypsin, kallikrein, plasmin, proteinase K, trypsin) by sulfonylation of a functional group in the active center of the enzyme. AEBSF (LD50 oral, mouse 2.8 g/kg) is a substantially less toxic substitute for PMSF and DFP. It is highly water-soluble and its solutions have an acidic pH. At this pH, stability is high. AEBSF is inactivated at 37°C by 50 % after 5 hours. We recommend storage of the stock solution (e. g. 20 mM or 100 mM in water or buffer) at -20°C (stable for up to 2 months; at +4°C approx. 1 week). It should be added to the assay buffers just before use at a final concentration of 0.1 - 2 mM.
Hazard pictograms:
WGK: 1
Signal Word: Danger
GHS Symbols: GHS05
H Phrases: H314
P Phrases: P280, P305+P351+P338, P310
CS: 29214900