Maximum Control and Transparency in Laboratory Dialysis
The proven XDB™ Dialysis Box from scienova is now available in an enhanced version:
XDB™ DaVis – featuring integrated real-time monitoring and control for maximum process safety, efficiency, and reliability in laboratory dialysis.
Through continuous measurement of conductivity and temperature, you can keep track of your dialysis processes at all times.
Monitoring up to 96 samples simultaneously saves time and improves reproducibility.
Key Features of XDB™ DaVis
- Real-Time Monitoring – continuous measurement of conductivity and temperature for maximum process control
- High Efficiency – automatic indication of the optimal buffer exchange time minimizes unnecessary waiting periods
- Process Safety – instant alerts when threshold values are exceeded enhance reliability
- High Reproducibility – stable measurement conditions and precise logging of all parameters
- Compatible & Flexible – SBS format, ideal for automated systems and high-throughput workflows
- Easy Operation – USB-powered, plug-and-measure principle, no additional hardware required
- Simplified Data Management – local CSV/XLS export, no cloud connection necessary
- Future-Proof – free software updates and continuous product development
Real-time visibility of process data
Representation of Dialysis Efficiency and Calculation of Time Effort Based on an Example:
In the XDB™, 9.6 ml of a potassium chloride-bovine serum albumin solution (KCl-BSA solution) with a concentration of 2 M KCl and 2 mg/ml BSA was dialyzed against 750 ml of distilled water. The dialysis was performed in 12 MD100 cartridges (cut-off 6-8 kDa), with each cartridge containing 8 × 100 µl KCl-BSA solution on a magnetic stirrer, supplemented by a conductivity sensor in the buffer reservoir. The blue line represents the measured conductivity (EC) of the diffused KCl, while the dashed red line shows the calculated percentage of remaining KCl in the dialyzers. The red dots indicate the percentage of BSA in the dialyzer, measured via optical density at 280 nm in a spectrometer. All data are based on three independent trials and are presented with mean values and standard deviations.
Total Duration:
The entire process takes approximately 51 minutes.
- Pipetting the Samples: Using an 8-channel pipette to fill the dialysis cartridges with 96 samples, filling the buffer reservoir, and placing the samples into the dialysis box. Duration: approx. 2 minutes.
- Dialysis Time: Dialysis until equilibrium is reached. Duration: approx. 45 minutes.
- Sample Extraction: Removing the cartridge insert and extracting the samples using an 8-channel pipette. Duration: approx. 2 minutes.
Result:
The initial concentration of 2 M KCl in the samples is reduced to approximately 25 mM. By repeating the dialysis through buffer exchange, the KCl concentration in the samples decreases to 0.33 mM.
Typical Applications
- Equilibrium dialysis (e.g. protein binding studies)
- Free hormone analysis (cortisol, testosterone, FT3/FT4)
- Buffer exchange and desalting
- Dialysis of proteins, peptides, and RNA
Scope of Delivery
XDB™ DaVis Box | XDB™ Sensor CT | DaVis Software | Calibration solution | USB-cable | user manual
