Micro Dialyzer

Sample dialysis – easy & efficient

The Xpress Dialyzers are ready-to-use dialysis systems for small sample volumes. They enable a very fast and efficient purification of protein, RNA and DNA samples. Compared to ultrafiltration and chromatography, the dialysis cartridges have an excellent sample recovery, offer gentle sample treatment and are very easy to handle.
The unique geometry of the scienova dialysis cartridges, the optimal ratio of the membrane surface to the sample volume and at the same time a minimal diffusion path, significantly reduces exchange and interaction processes (dialysis time).

Designed in a practical cartridge format, the scienova Xpress Dialyzers are concepted “ready-to-use” and allow easy handling by standard laboratory pipettes.
The scienova Xpress Dialyzers are compatible with the microtiter plate standard (SBS) and can be used in most pipetting and laboratory automation systems.
The dialyzers are therefore suitable for routine laboratory work (e. g. cleaning of protein samples after tryptic digestion before LC/MS analyses) or high-throughput screening (HTS) and scouting experiments.

Interaction and binding studies, for example in the development of active pharmaceutical ingredients or in the diagnostic determination of free hormones from blood serum, can be realized with equilibrium dialysis.

With this modern dialysis technology, you not only conserve your resources, but also achieve a completely new quality of cost-effectiveness compared to conventional separation processes.

applications

  • protein purification:
    – desalination, buffering, removal of dyes and concentration of samples
  • renaturation / denaturation of proteins:
    – removal of urea, removal of guanidine hydrochloride
  • cleaning of nanoparticles
  • equilibrium dialysis
    – hormone determination
    – interaction and binding studies of active substances

advantages

  • high sample throughput with simultaneous reduction of analysis and sample preparation times by 2 to 12 times
  • high yield with up to 98% sample recovery
  • optimized for use in liquid handling systems according to the microtitre plate standard
  • easy sample loading and sample extraction with standard laboratory pipettes
  • autoclavable

 

 Xpress Dialyzer Family

membrane: low-binding regenerated cellulose  I  cut-off (MWCO): 2, 3.5, 6-8, 20 kDa  I  temperature: 1-60°C  I  pH-value: 4-8
Bio-Xell ® I cut-off (MWCO) 140 kDa I temperature: 1-60°C I pH-value: 5-8

MD100
sample volume 10-100 μl
deep-well plates 96-well

ED300
sample volume 50-300 μl
deep-well plates 96-well

MD1000
sample volume 150-1000 μl
deep-well plates 48-well

MD300
sample volume 50-300 μl
deep-well plates 96-well

XDR
sample volume 1 ml – 19 ml
deep-well plates 6-well

 

Xpress Micro Dialyzer 100

  • sample volumes between 10 and 100 µl
  • cut-off 2 I 3.5 | 6-8 | 12-14 I 20 I 140 kDa
  • SBS compatible
  • autoclavable
  • up to 98% sample recovery
  • protein and peptide sample purification
  • sample renaturation
  • re-buffering of protein samples
  • in vitro translation
  • enzyme activity studies
  • binding studies (hormones, drugs)

 

Xpress Micro Dialyzer 300

  • sample volumes between 50 and 300 µl
  • cut-off 2 I 3.5 | 6-8 | 12-14 I 20 I 140 kDa
  • SBS compatible
  • autoclavable
  • protein and peptide sample purification
  • sample renaturation
  • re-buffering of protein samples
  • in vitro translation

Xpress Equilibrium Dialyzer

  • sample volumes between 50 and 300 µl
  • cut-off 2 I 3.5 | 6-8 | 12-14 I 20 I 140 kDa
  • 2-channel system allows easy sample handling and buffer exchange directly out of the deep well plate (automated process)
  • SBS compatible
  • autoclavable
  • protein and peptide sample purification
  • sample renaturation
  • re-buffering of protein samples
  • in vitro translation
  • enzyme activity studies
  • binding studies (hormones, drugs)

Xpress Mini Dialyzer

  • sample volumes between 150 and 1000 µl
  • cut-off 2 I 3.5 | 6-8 | 12-14 I 20 I 140 kDa
  • 2-channel system allows easy sample handling and buffer exchange directly out of the deep well plate (automated process)
  • protein and peptide sample purification
  • sample renaturation
  • re-buffering of protein samples
  • in vitro translation
  • enzyme activity studies

 

Xpress Dialysis Reactor

  • sample volumes from 1 ml – 19 ml
  • cut-off 2 I 3.5 | 6-8 | 12-14 I 20 kDa

 

Xpress Magnetic Mixing Dialysis Box

  • even higher dialysis efficiency due to buffer circulation during dialysis
  • complete set with 12 cartridges of Xpress Mirco Dialyzer 100 / 300 or Xpress Equilibrium Dialyzer
  • configured for immediate use on a magnetic stirrer
  • protein and peptide sample purification
  • sample renaturation
  • re-buffering of protein samples

GridKit48

  • tool for larger buffer volume
  • easy buffer change directly in the deep well plate through positioning grid
  • optimized and tested for use in liquid handling systems
  • protein and peptide sample purification
  • sample renaturation
  • re-buffering of protein samples
  • in vitro translation
  • enzyme activity studies
  • binding studies (hormones, drugs)

 

 

 

 

 

     
"Development of a utilization strategy for scienova's Xpress Dialyzer products "funded by the Free State of Thuringia from the European Social Fund